Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in growth factor levels in human wound fluid.

V M Dvonch1, R J Murphey, J Matsuoka

  • 1Department of Ophthalmology, College of Medicine, University of South Florida, Tampa.

Surgery
|July 1, 1992
PubMed
Summary

Platelet-derived growth factor (PDGF) and monocyte/macrophage-derived growth factor (MDGF) levels change over time after surgery, supporting wound repair models. MDGF may be crucial for healing, acting on cells unresponsive to PDGF AA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Randomized phase III trial of treatment duration for oral uracil and tegafur plus leucovorin as adjuvant chemotherapy for patients with stage IIB/III colon cancer: final results of JFMC33-0502.

Annals of oncology : official journal of the European Society for Medical Oncology·2015
Same author

Treatment of impending pathological fractures of the femur with flexible intramedullary nails.

Orthopedics·2014
Same author

Carpal-tarsal osteolysis.

Orthopedics·2014
Same author

Blood-brain barrier disruption is more severe in neuromyelitis optica than in multiple sclerosis and correlates with clinical disability.

The Journal of international medical research·2012
Same author

Spontaneously complete regression of pseudolymphoma of the remnant pancreas after pancreaticoduodenectomy.

Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]·2012
Same author

Upregulation of cancer-associated myofibroblasts by TGF-β from scirrhous gastric carcinoma cells.

British journal of cancer·2011

Area of Science:

  • Wound healing research
  • Cell signaling in tissue repair

Background:

  • Platelet-derived growth factor (PDGF) is implicated in wound healing.
  • Human wound fluid contains PDGF AA and monocyte/macrophage-derived growth factor (MDGF) post-surgery.

Purpose of the Study:

  • To analyze the temporal changes in PDGF and MDGF levels and activity in human wound fluid.
  • To investigate the specific role of MDGF in wound healing.

Main Methods:

  • Assayed PDGF AA concentration using Western blot.
  • Determined chemotactic and mitogenic potential of wound fluid components.
  • Assayed MDGF biologic activity on cell lines unresponsive to PDGF AA.

Main Results:

  • PDGF AA and MDGF concentrations and biologic activity peaked immediately post-surgery, declining within 24 hours.

Related Experiment Videos

  • MDGF chemotactic activity also peaked early and decreased over 24 hours.
  • MDGF demonstrated biologic activity on cell lines unresponsive to PDGF AA.

    • Conclusions:

    • Observed changes in PDGF and MDGF levels support a cascade model of wound repair.
    • MDGF's distinct activity suggests a significant, potentially independent, role in wound healing.