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Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds
Published on: October 20, 2012
Changes in growth factor levels in human wound fluid
V M Dvonch1, R J Murphey, J Matsuoka
1Department of Ophthalmology, College of Medicine, University of South Florida, Tampa.
Insights
Platelet-derived growth factor (PDGF) and monocyte/macrophage-derived growth factor (MDGF) levels change over time after surgery, supporting wound repair models. MDGF may be crucial for healing, acting on cells unresponsive to PDGF AA.
Area of Science:
- Wound healing research
- Cell signaling in tissue repair
Background:
- Platelet-derived growth factor (PDGF) is implicated in wound healing.
- Human wound fluid contains PDGF AA and monocyte/macrophage-derived growth factor (MDGF) post-surgery.
Purpose of the Study:
- To analyze the temporal changes in PDGF and MDGF levels and activity in human wound fluid.
- To investigate the specific role of MDGF in wound healing.
Main Methods:
- Assayed PDGF AA concentration using Western blot.
- Determined chemotactic and mitogenic potential of wound fluid components.
- Assayed MDGF biologic activity on cell lines unresponsive to PDGF AA.
Main Results:
- PDGF AA and MDGF concentrations and biologic activity peaked immediately post-surgery, declining within 24 hours.
- MDGF chemotactic activity also peaked early and decreased over 24 hours.
- MDGF demonstrated biologic activity on cell lines unresponsive to PDGF AA.
Conclusions:
- Observed changes in PDGF and MDGF levels support a cascade model of wound repair.
- MDGF's distinct activity suggests a significant, potentially independent, role in wound healing.
Background:
It has been suggested that platelet-derived growth factor (PDGF) plays a central role in wound healing. Analysis of human wound fluid revealed the presence of PDGF AA (30 kd) and monocyte/macrophage-derived growth factor (MDGF) (12 to 14 kd) in the immediate postoperative period.
Methods:
The amount of PDGF AA present was assayed by Western blot analysis. The chemotactic and mitogenic potential of purified wound fluid containing PDGF AA and MDGF was determined on a responsive cell line. The biologic activity of MDGF was assayed with a cell line that is unresponsive to the PDGF AA found in wound fluid.
Results:
Both the concentration and the biologic activity were highest in the immediate postoperative period and declined to negligible levels by 24 hours after surgery. The chemotactic activity of MDGF was highest in the immediate postoperative period and declined during the first 24 hours in a manner similar to that of the combined PDGF AA and MDGF activity.
Conclusions:
These data demonstrate the changing levels of PDGF and MDGF in human wound fluid over time, supporting the cascade model of wound repair. By demonstrating that MDGF acts on cell lines unresponsive to the PDGF AA found in wound fluid, these data suggest that MDGF may also play an important role in wound healing.
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