I-DIRT, a general method for distinguishing between specific and nonspecific protein interactions
Alan J Tackett1, Jeffrey A DeGrasse, Matthew D Sekedat
1The Rockefeller University, New York, NY 10021, USA.
Journal of Proteome Research
|October 11, 2005
Abstract:
Isolation of protein complexes via affinity-tagged proteins provides a powerful tool for studying biological systems, but the technique is often compromised by co-enrichment of nonspecifically interacting proteins. We describe a new technique (I-DIRT) that distinguishes contaminants from bona fide interactors in immunopurifications, overcoming this most challenging problem in defining protein complexes. I-DIRT will be of broad value for studying protein complexes in biological systems that can be metabolically labeled.
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