Related Experiment Video
Updated: Jul 22, 2026
Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
Published on: February 14, 2016
A bovine protocol for training professionals in preimplantation genetic diagnosis using polymerase chain reaction
Carlos Gilberto Almodin1, Antonio Fernandes Moron, Luiz Kulay
1Materbaby--Reprodução Humana e Genética, Maringá, Paraná, Brazil. almodin@materbaby.com.br
Objective:
To develop a bovine protocol for training in preimplantation genetic diagnosis (PGD) using PCR.
Design:
Randomized study.
Setting:
Human reproduction PCR laboratory.
Patient(S):
Cow ovaries obtained from slaughterhouses.
Intervention(S):
The ovaries were punctured and the oocytes were matured and submitted to in vitro fertilization. On the third day after fertilization, the embryos were biopsied and 1-2 blastomeres removed. A blastomere and the rest of the embryo were submitted to PCR for sex determination.
Main Outcome Measure(S):
Establishment of a possible training protocol.
Result(S):
A total of 50 embryos and 50 biopsied blastomeres were submitted to DNA amplification for sexing. Of the 50 embryos, 41 (82%) achieved successful DNA amplification and 9 (18%) did not. Of the 50 biopsies, 31 (62%) amplified and 19 (38%) did not. In 27 (65.9%) of the 41 embryos with DNA amplification, sex was identified as female and in 14 (34.1%) as male. In 40 cases (80%) amplification and sex determination were successful in both embryos and blastomeres. Sex was identical in all these cases.
Conclusion(S):
This training model seems to be useful in identifying mistakes and difficulties and improving the professional's performance in the various stages of preimplantation genetic diagnosis.
