Successful sperm storage for 28 years
Related Experiment Video
Updated: Aug 15, 2026
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
Published on: July 6, 2009
Joseph Feldschuh1, James Brassel, Nancy Durso
1Idant Laboratories, New York, New York, USA. jfeldschuh@daxor.com
Objective:
To report on two instances of successful long-term cryopreservation resulting in live births.
Design:
Case report.
Setting:
The patient stored his sperm at a private sperm banking facility, and his partner underwent artificial insemination at an obstetrics/gynecology practice for one pregnancy and at a fertility center for the other.
Patient(S):
One man who stored his sperm before treatment for cancer.
Intervention(S):
Storage of sperm under liquid nitrogen in a carousel canister system. Intrauterine insemination in which the semen was thawed, washed with human tubal fluid (HTF), and inseminated.
Main Outcome Measure(S):
Successful pregnancy resulting in live birth.
Result(S):
Artificial insemination with semen cryopreserved for 21 and 28 years resulted in two live births.
Conclusion(S):
This case report describes the to-date longest known successful cryopreservation of sperm, with two live births resulting from IUI. Successful long-term semen storage can be very beneficial for men facing impaired fertility or sterility early in life, so that they have sufficient time to make appropriate family planning decisions.
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