Related Experiment Video
Updated: Aug 15, 2026

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
Published on: March 2, 2017
Successful pregnancy and delivery after transfer of a single blastocyst derived from a vitrified mature human oocyte
Koichi Kyono1, Kohei Fuchinoue, Akiko Yagi
1Department of Gynecology and Urology, Ladies Clinic Kyono, Miyagi, Japan. info@ivf-kyono.or.jp
Objective:
To describe the first case of delivery after the transfer of a single blastocyst derived from a vitrified mature human oocyte.
Design:
Case report.
Setting:
Private assisted reproduction clinic.
Patient(S):
A normal 31-year-old woman.
Intervention(S):
An unsuccessful attempt was made to extract sperm from the patient's nonobstructive azoospermic husband by testicular sperm extraction.
Main Outcome Measure(S):
Transfer of single blastocyst derived from vitrified human oocyte and donor sperm.
Result(S):
A healthy male neonate weighing 3000 g was born.
Conclusion(S):
Vitrification is a useful method of preserving mature human oocytes and has the advantage of time-effectiveness, simplicity, cost reduction, and no need for devices such as programmable freezers.
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