Related Experiment Video

Updated: Aug 15, 2026

Quantifying the Level of 8-oxo-dG Using ELISA Assay to Evaluate Oxidative DNA Damage in MCF-7 Cells
05:13

Quantifying the Level of 8-oxo-dG Using ELISA Assay to Evaluate Oxidative DNA Damage in MCF-7 Cells

Published on: May 24, 2024

Does measurement of oxidative damage to DNA have clinical significance?

Marcus S Cooke1, Ryszard Olinski, Mark D Evans

  • 1Radiation and Oxidative Stress Group, Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester Royal Infirmary, University Hospitals of Leicester NHS Trust, Leicester, LE2 7LX, UK. msc5@le.ac.uk

Clinica Chimica Acta; International Journal of Clinical Chemistry
|October 11, 2005
PubMed

Insights

Oxidative DNA damage, a result of cell metabolism and stress, is linked to many diseases. Further research into biomarkers could make them valuable clinical tools for disease diagnosis and treatment.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Cellular metabolism naturally produces oxidative DNA damage.
  • Oxidative stress, from external insults, can increase DNA damage levels.
  • Elevated DNA damage is observed in numerous diseases, suggesting a role in their development.

Purpose of the Study:

  • To review the hypothesis linking oxidative DNA damage to disease aetiology.
  • To explore the mechanisms through which oxidative DNA damage contributes to disease.
  • To assess the potential clinical utility of oxidative DNA damage biomarkers.

Main Methods:

  • Literature review of studies on oxidative DNA damage and disease.
  • Analysis of mechanisms connecting DNA damage to pathological processes.
  • Evaluation of current and potential biomarkers for oxidative DNA damage.

Main Results:

  • Oxidative DNA damage is a common occurrence in cellular processes.
  • Evidence suggests a significant role for oxidative DNA damage in the etiology of various diseases.
  • Biomarkers for oxidative DNA damage show promise but require further validation.

Conclusions:

  • The hypothesis linking oxidative DNA damage to disease aetiology is supported by current evidence.
  • Understanding the mechanisms of damage is crucial for disease intervention.
  • Validated biomarkers of oxidative DNA damage could become important clinical tools.

More Related Videos

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues14:12

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

Published on: August 1, 2015

Comet Assay as an Indirect Measure of Systemic Oxidative Stress08:23

Comet Assay as an Indirect Measure of Systemic Oxidative Stress

Published on: May 22, 2015

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...