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Related Experiment Videos

Optimizing double emulsion process to decrease the burst release of protein from biodegradable polymer microspheres.

J X Zhang1, D Chen, S J Wang

  • 1Institute of Polymer Science, Zhejiang University, Hangzhou, P.R. China.

Journal of Microencapsulation
|October 11, 2005
PubMed
Summary

Optimizing biodegradable polymer microsphere formulations significantly reduced burst release of bovine serum albumin (BSA). Non-buffered inner phases and probe sonication yielded the best results for controlled drug delivery.

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Area of Science:

  • Materials Science
  • Pharmaceutical Technology
  • Biomedical Engineering

Background:

  • Biodegradable polymer microspheres are crucial for controlled drug delivery.
  • Minimizing initial burst release of encapsulated drugs like bovine serum albumin (BSA) is a key challenge.
  • Double emulsion methods are commonly used for microsphere preparation.

Purpose of the Study:

  • To optimize process parameters for reducing BSA burst release from biodegradable polymer microspheres.
  • To investigate the impact of inner and outer aqueous phase compositions and emulsification techniques.
  • To evaluate different polymer types and additives for improved microsphere performance.

Main Methods:

  • Optimization of inner and outer aqueous phase compositions.

Related Experiment Videos

  • Comparison of emulsification techniques: probe sonication, homogenization, mechanical stirring.
  • Evaluation of additives (NaCl, glucose, mannitol) and surfactants (Tween 80, PVP, PVA).
  • Assessment of polymer copolymers including L-lactide (LLA) with dimethyl trimethylene carbonate (DTC) or trimethylene carbonate (TMC).

    • Main Results:

    • A non-buffered inner aqueous phase reduced BSA burst release by up to 14%.
    • Probe sonication for primary emulsion formation resulted in insignificant burst effect.
    • Using 0.1% Tween 80 in the outer phase reduced burst to 2.7%, though yield was low (52.1%).
    • Additives like NaCl and glucose increased burst release at specific concentrations and affected BSA entrapment.
    • PVP and Tween 80 as surfactants diminished burst release compared to PVA.
    • Microspheres made with DTC or TMC copolymers showed insignificant burst effect.

    Conclusions:

    • Process parameter optimization, particularly non-buffered inner phases and probe sonication, is effective in minimizing burst release of BSA.
    • Tween 80 and PVP are suitable surfactants for reducing burst release.
    • DTC and TMC copolymers offer potential for developing microspheres with negligible burst release.