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Published on: October 4, 2019
Profiling of differentially expressed genes in LRRC4 overexpressed glioblastoma cells by cDNA array
Qiu-Hong Zhang1, Ming-Hua Wu, Li-Li Wang
1Cancer Research Institute, Central South University, Changsha 410078, China.
Abstract:
Our previous study has shown that LRRC4 is a novel member of the leucine-rich repeat (LRR) superfamily and has the potential to suppress brain tumor growth. In order to further analyze the functions of LRRC4 on the maintenance of normal function and suppression of tumorigenesis in the central nervous system, we investigated alterations in gene expression related to neurobiology by the Atlas array in two inducible dual-stable LRRC4-overexpressing cell lines. Seventeen of 588 genes spotted on the Atlas membrane showed altered expression levels in LRRC4 transfected U251MG Tet-on cells, which are involved in cell proliferation and cell cycle progression, tumor invasion and metastasis, and neurotransmitter synthesis and release. In addition, cell invasion assay results showed that LRRC4 can inhibit the U251MG cell migration. These studies represent the first cDNA array analysis of the effects of LRRC4 on the involvement of different neurobiological genes in U251MG glioblastoma cells and provide new insights into the function of LRRC4 in glioma.
Insights
Leucine-rich repeat-containing protein 4 (LRRC4) suppresses brain tumor growth by altering neurobiological gene expression. This study reveals LRRC4
Area of Science:
- Neuro-oncology
- Molecular Biology
- Gene Expression Analysis
Background:
- Leucine-rich repeat-containing protein 4 (LRRC4) is a novel leucine-rich repeat (LRR) superfamily member.
- Previous research indicated LRRC4's potential in suppressing brain tumor growth.
- Understanding LRRC4's role in central nervous system (CNS) function and tumorigenesis requires further investigation.
Purpose of the Study:
- To investigate the functional impact of LRRC4 on neurobiological gene expression.
- To analyze LRRC4's effects on glioblastoma cell line U251MG.
- To explore LRRC4's role in maintaining normal CNS function and suppressing tumorigenesis.
Main Methods:
- Utilized Atlas array technology to analyze gene expression in LRRC4-overexpressing U251MG Tet-on cells.
- Investigated alterations in 588 neurobiology-related genes.
- Performed cell invasion assays to assess LRRC4's effect on cell migration.
Main Results:
- Seventeen out of 588 genes exhibited altered expression levels in LRRC4-transfected cells.
- Affected genes are involved in critical cellular processes including proliferation, cell cycle, tumor invasion, metastasis, and neurotransmitter synthesis/release.
- LRRC4 demonstrated an inhibitory effect on U251MG cell migration.
Conclusions:
- This study represents the first cDNA array analysis of LRRC4's effects on neurobiological genes in U251MG glioblastoma cells.
- LRRC4 influences key pathways related to glioblastoma progression.
- LRRC4 provides new insights into glioma pathogenesis and potential therapeutic strategies.
