Related Experiment Video
Updated: Aug 11, 2026
Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
Asthma inhalers and subsurface enamel demineralisation: an in situ pilot study
R Tootla1, G Kotru, M A Connolly
1Dept. Paediatric Dentistry, Leeds Dental Institute, England.
Aim:
The purpose of this pilot study was to identify the subsurface enamel demineralising potential of two possible acidogenic lactose-based powders and their corresponding generic pump inhalers.
Methods:
Ten healthy non-asthmatic adults participated in a 5- leg randomised crossover study including a 10% sucrose control. A twice-daily 400 microg dose of inhaler was applied in vitro to a demineralised enamel slab on the buccal flange of a mandibular removable appliance before in situ placement for 14 days each. Lesion parameters were determined using transverse microradiography and digitised image analysis.
Results:
Minimal demineralisation occurred with sucrose, both pump and one powder inhaler. The remaining powder was associated with remineralisation (p = 0.29). Overall, mean lesion depth increased (p = 0.12).
Conclusion:
Asthma inhalers failed to demonstrate a significant acidogenic/cariogenic effect.
