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Published on: February 6, 2018
Molecular characterisation of the caprine (Capra hircus) lymphocyte function-associated antigen-1 alpha
Thomas Fett1, Laurent L M Zecchinon, Etienne A Baise
1Pathology Department, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium. thomas.fett@ulg.ac.be
Background:
Lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, alpha L beta 2) is required for many cellular adhesive interactions during the immune response.
Methods:
We used SMART RACE technology to obtain caprine CD11a 5'- and 3'-ends and RT-PCR to amplify the full-length CDS.
Results:
The Capra hircus CD11a-encoding cDNA was sequenced and compared with its human, murine, rat, bovine and ovine counterparts. Despite some focal differences, it shares all the main characteristics of its known mammalian homologues.
Conclusion:
Therefore, along with the caprine CD18-encoding cDNA, which has been available for a few months, the sequence data revealed here will allow the Capra hircus LFA-1 expression in vitro as a tool to explore the specificities of inflammation in the caprine species.
