Related Experiment Video
Updated: Aug 15, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
Published on: September 20, 2018
INI1/hSNF5/BAF47 represses c-fos transcription via a histone deacetylase-dependent manner
Xuefang Pan1, Lei Zhai, Ru Sun
1Institute of Genetics and Cytology, School of Life Science, Northeast Normal University, Changchun, 130024 Jilin, China.
Abstract:
INI1/hSNF5/BAF47 is a core component of the hSWI/SNF ATP-dependent chromatin-remodeling complex. It has been suggested that INI1/hSNF5/BAF47 contributes to the regulation of many genes. In this report, we showed that the overexpression of INI1/hSNF5/BAF47 repressed c-fos promoter activity and endogenous c-fos transcription in 293T cells, and the siRNA targeting INI1/hSNF5/BAF47 (siINI1) reversed the inhibitory effect. Histone deacetylation by histone deacetylases (HDACs) was necessary for the repression of c-fos transcription by INI1/hSNF5/BAF47. HDAC and INI1/hSNF5/BAF47 functioned together to suppress c-fos transcription. ChIP experiments demonstrated that INI1/hSNF5/BAF47 could be recruited to the region of c-fos promoter to reduce histone acetylation. Altogether, these data show that INI1/hSNF5/BAF47 represses c-fos transcription via a histone deacetylase (HDAC)-dependent manner.
Related Concept Videos
Co-activators and Co-repressors
Co-activators and Co-repressors
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Histone Modification
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Histone Modification
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
RNA Polymerase II Accessory Proteins

