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Published on: May 22, 2018
Protective effect of Polygonum multiflorum Thunb on amyloid beta-peptide 25-35 induced cognitive deficits in mice
Min-Young Um1, Won-Hee Choi, Ji-Yun Aan
1Food Function Research Division, Korea Food Research Institute, Seongnam 463-746, Republic of Korea.
Abstract:
Amyloid beta protein (Abeta) may be neurotoxic during the progression of Alzheimer's disease by eliciting oxidative stress. This study was designed to determine the effect of Polygonum multiflorum Thunb water extract (PWE) on Abeta25-35-induced cognitive deficits and oxidative stress in mice. Mice were fed experimental diets comprising either 0.5 or 1% PWE for 4 weeks, and then received a single intracerebroventricular (i.c.v.) injection of Abeta25-35 (10 microg/mouse). Behavioral changes in the mice were evaluated using passive avoidance and water-maze tests. The consumption of PWE significantly ameliorated the cognitive deficits caused by i.c.v. injection of Abeta25-35. The Abeta25-35 treatment accelerated the lipid peroxidation, and PWE attenuated the Abeta-induced increase in brain levels of thiobarbituric acid reactive substances. There was an increase in glutathione peroxidase activity in PWE-treated groups. The acetylcholinesterase activity in the brain and serum was lower in PWE supplemented groups than in the only Abeta-injected group. These findings suggest that PWE exerts a preventive effect against cognitive deficits induced by Abeta25-35 accumulation in Alzheimer's disease, and that this effect is mediated by the antioxidant properties of PWE.
Insights
Polygonum multiflorum water extract (PWE) prevents cognitive decline and oxidative stress in Alzheimer's disease models. PWE demonstrates antioxidant properties, offering a potential therapeutic avenue.
Area of Science:
- Neuroscience
- Pharmacology
- Biochemistry
Background:
- Alzheimer's disease (AD) is characterized by amyloid beta protein (Abeta) accumulation, leading to neurotoxicity and oxidative stress.
- Amyloid beta 25-35 (Abeta25-35) peptide is known to induce cognitive deficits and oxidative damage in experimental models.
Purpose of the Study:
- To investigate the neuroprotective effects of Polygonum multiflorum Thunb water extract (PWE) against Abeta25-35-induced cognitive impairment and oxidative stress in mice.
- To elucidate the underlying mechanisms of PWE's action, focusing on its antioxidant properties.
Main Methods:
- Mice were administered diets containing 0.5% or 1% PWE for 4 weeks prior to intracerebroventricular (i.c.v.) injection of Abeta25-35.
- Cognitive function was assessed using passive avoidance and water-maze tests.
- Biochemical markers of oxidative stress, including lipid peroxidation and antioxidant enzyme activity (glutathione peroxidase), were measured. Acetylcholinesterase activity was also assessed.
Main Results:
- PWE supplementation significantly ameliorated Abeta25-35-induced cognitive deficits in both behavioral tests.
- PWE attenuated the increase in thiobarbituric acid reactive substances (TBARS), a marker of lipid peroxidation, in the brain.
- Glutathione peroxidase activity was elevated in PWE-treated groups, and acetylcholinesterase activity was reduced.
Conclusions:
- Polygonum multiflorum water extract exhibits significant preventive effects against Abeta25-35-induced cognitive deficits and oxidative stress.
- The neuroprotective effects of PWE are attributed to its potent antioxidant properties, making it a potential therapeutic agent for Alzheimer's disease.
