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Effects of ferulic acid on E-selectin expression in activated endothelial cell and leukocyte-endothelial cell
Xiao-lan Wang1, Xiao-hui Hu, Ming-en Lü
1First Affiliated Hospital of Suzhou University, Jiangsu Institute of Hematology, Suzhou 215006, China.
Aim:
To study the effects of ferulic acid (FA) on E-selectin expression in human umbilical vein endothelial cells (HUVECs) activated by lipopolysaccharide and leukocyte-endothelial cell adhesion.
Methods:
The effects of FA on E-selectin and E-selectin mRNA expression were determined by flow cytometry and reverse transcription polymerase chain reaction. The effect of FA on HL60-HUVEC adhesion was evaluated with the method of staining the cells by Rose Bengal.
Results:
The expression of E-selectin and E-selectin mRNA were down regulated by FA (0.62 and 0.41 mmol x L(-1), respectively). HL60 cells adhered to activated HUVECs were also reduced by FA (0.62 and 0.41 mmol x L(-1), respectively).
Conclusion:
FA can inhibit the expression of E-selectin and E-selectin mRNA and HL60-HUVEC adhesion. This may contribute to its protective effect against ischemia-reperfusion injury.
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