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A reliable RT-PCR-ELISA method for the detection of infectious pancreatic necrosis virus (IPNV) in farmed rainbow

S A Milne1, S Gallacher, P Cash

  • 1Department of Molecular and Cell Biology, School of Medical Sciences, University of Aberdeen, I.M.S Building, Foresterhill, Aberdeen AB25 2ZD, Scotland. s.a.milne@abdn.ac.uk

Journal of Virological Methods
|October 14, 2005
PubMed

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...