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Updated: Aug 15, 2026

Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples
Published on: June 14, 2024
Leptospirosis after recreational exposure to water in the Yaeyama islands, Japan
Masashi Narita1, Shigeki Fujitani, David A Haake
1University of Pittsburgh, Internal Medicine Department, Pittsburgh, Pennsylvania 15232, USA. naritam@upmc.edu
Abstract:
Leptospirosis is a global zoonotic disease with a variety of clinical manifestations. We report an outbreak of leptospirosis in the Yaeyama Islands, Japan, in the summer of 1999 associated with heavy rainfall. Fourteen people were diagnosed with leptospirosis and required hospitalization. All cases were found to have exposure to contaminated soil or water. A history of recreational activities involving water sports was more frequent (71%) than occupational risk factors related to agriculture or construction (29%). Fever was the primary symptom in all cases, followed by chills (93%), headache (86%), myalgias (57%) and conjunctival suffusion (57%). All cases were successfully treated with antimicrobial therapy except one patient who improved spontaneously. Jarisch-Herxheimer reactions were seen in six cases (43%). The increasing incidence of leptospirosis related to recreational sports is an important public health problem in resort areas. A high-index of suspicion, early treatment, and prevention are crucial in this latently endemic area.
Insights
An outbreak of leptospirosis in Japan was linked to contaminated water and soil following heavy rainfall. Recreational activities were a significant risk factor, highlighting the need for public health awareness and prevention in endemic areas.
Area of Science:
- Zoonotic diseases
- Infectious disease epidemiology
- Public health
Background:
- Leptospirosis is a global zoonotic disease with diverse clinical presentations.
- Outbreaks are often associated with environmental factors like heavy rainfall.
- The Yaeyama Islands, Japan, are a known endemic area.
Purpose of the Study:
- To report on a leptospirosis outbreak in the Yaeyama Islands in 1999.
- To identify risk factors and clinical manifestations.
- To emphasize public health implications for recreational areas.
Main Methods:
- Retrospective case analysis of hospitalized patients.
- Epidemiological investigation of exposure routes (soil, water).
- Clinical symptom and treatment outcome assessment.
Main Results:
- Fourteen hospitalized cases of leptospirosis were diagnosed.
- Exposure to contaminated soil or water was confirmed in all cases.
- Recreational water sports (71%) were a more frequent exposure than occupational (29%).
- Common symptoms included fever, chills, headache, myalgias, and conjunctival suffusion.
- Antimicrobial therapy was effective; Jarisch-Herxheimer reactions occurred in 43%.
Conclusions:
- Leptospirosis outbreaks can be associated with heavy rainfall and contaminated environments.
- Recreational activities pose an increasing risk for leptospirosis transmission.
- Early diagnosis, prompt treatment, and preventative measures are critical in endemic regions.
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