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Related Experiment Videos

Lactococcus lactis release from calcium alginate beads.

C P Champagne1, C Gaudy, D Poncelet

  • 1Centre de Recherche et de Développement sur les Aliments, Agriculture Canada, Saint-Hyacinthe, Québec.

Applied and Environmental Microbiology
|May 1, 1992
PubMed
Summary
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Immobilized Lactococcus lactis in alginate beads released cells during milk fermentation. Ethanol treatment effectively reduced viable cell release while maintaining acidification activity.

Area of Science:

  • Food Microbiology
  • Biotechnology
  • Dairy Science

Background:

  • Lactococcus lactis is crucial for milk fermentation.
  • Immobilization in calcium alginate beads is used to retain starter cultures.
  • Controlling cell release from immobilized cultures is important for product safety and consistency.

Purpose of the Study:

  • To investigate cell release from Lactococcus lactis immobilized in calcium alginate beads during milk fermentation.
  • To evaluate methods for reducing viable cell release while maintaining fermentation activity.

Main Methods:

  • Cells of Lactococcus lactis were immobilized in calcium alginate beads.
  • Beads were reused for successive milk fermentations.
  • Various treatments were applied to reduce cell release, including rinsing, poly-L-lysine (PLL) coating, double PLL-alginate coating, ethanol immersion, and hot CaCl2 treatment.

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Main Results:

  • Free cell numbers increased with bead reuse, reaching 3 x 10(7) CFU/ml.
  • Rinsing or initial cell density did not affect cell release.
  • A double PLL-alginate coating reduced cell release by 50% but slowed acidification.
  • Ethanol treatment significantly reduced cell release and maintained acidification activity.
  • Aluminum nitrate and hot CaCl2 treatments were less effective than ethanol.

Conclusions:

  • Cell release from immobilized Lactococcus lactis is a concern during repeated milk fermentation.
  • Ethanol treatment of beads is a promising strategy to minimize viable cell release while preserving acidification capacity.
  • Surface cell removal via ethanol treatment offers a viable solution for starter culture applications.