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A ratiometric fluorescence sensor with broad dynamic range based on two pH-sensitive fluorophores.

Cheng-Gang Niu1, Xiao-Qin Gui, Guang-Ming Zeng

  • 1College of Environmental Science and Engineering, State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, P. R. China. cgniu@hnu.cn

The Analyst
|October 14, 2005
PubMed
Summary

This study introduces a new ratiometric fluorescence sensor for precise pH measurement across a wide range (pH 1.5-9.0). The sensor utilizes two pH-sensitive fluorophores for accurate and stable readings, offering an alternative for sensor development.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Chemical Sensing

Background:

  • Accurate pH monitoring is crucial across various scientific disciplines.
  • Existing pH sensors may have limitations in dynamic range or stability.
  • Ratiometric fluorescence sensing offers enhanced accuracy by using internal referencing.

Purpose of the Study:

  • To develop a novel ratiometric fluorescence sensor for broad-range pH measurement.
  • To co-polymerize two distinct pH-sensitive fluorophores onto a solid support.
  • To evaluate the sensor's performance characteristics for practical applications.

Main Methods:

  • Co-polymerization of N-allyl-4-(4'-methyl-piperazinyl)-1,8-naphthalimide (AMPN) and meso-5,10,15,20-tetra-(4-allyloxyphenyl)porphyrin (TAPP) with monomers on a silanized glass surface.

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  • Utilizing the pH-dependent fluorescence intensities of AMPN and TAPP as referencing indicators.
  • Characterization of sensor performance including dynamic range, selectivity, reproducibility, and stability.

    • Main Results:

    • The developed sensor successfully measures pH over a broad dynamic range of 1.5-9.0.
    • The sensor demonstrated satisfactory selectivity, reproducibility, and stability.
    • The ratiometric approach using two fluorophores proved effective for pH sensing.

    Conclusions:

    • A novel ratiometric fluorescence sensor for pH measurement has been successfully fabricated.
    • The sensor offers a reliable and stable method for monitoring pH across a wide range.
    • This work presents a new concept for developing broad-range ratiometric pH sensors.