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High-pressure, high-temperature bioreactor for comparing effects of hyperbaric and hydrostatic pressure on bacterial
C M Nelson1, M R Schuppenhauer, D S Clark
1Department of Chemical Engineering, University of California, Berkeley 94720-9989.
Applied and Environmental Microbiology
|May 1, 1992
Abstract:
We describe a high-pressure reactor system suitable for simultaneous hyperbaric and hydrostatic pressurization of bacterial cultures at elevated temperatures. For the deep-sea thermophile ES4, the growth rate at 500 atm (1 atm = 101.29 kPa) and 95 degrees C under hydrostatic pressure was ca. three times the growth rate under hyperbaric pressure and ca. 40% higher than the growth rate at 35 atm.