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Related Experiment Videos

Grating-coupled surface plasmon resonance: a cell and protein microarray platform.

Darryn W Unfricht1, Sara L Colpitts, Salvador M Fernandez

  • 1Department of Molecular and Cell Biology, University of Connecticut, Storrs, 06269, USA.

Proteomics
|October 14, 2005
PubMed
Summary

Grating-coupled surface plasmon resonance (GCSPR) offers accurate, multiplexed analysis of analytes with minimal sample. This label-free technology simultaneously detects various biomolecules and cellular components, advancing biological sample analysis.

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Area of Science:

  • Biophysics
  • Analytical Chemistry
  • Immunology

Background:

  • Surface plasmon resonance (SPR) is a label-free optical technique used to study biomolecular interactions.
  • Grating-coupling enhances SPR sensitivity and allows for miniaturization and multiplexing.
  • Accurate assessment of analytes in a multiplexed format requires sensitive and efficient detection methods.

Purpose of the Study:

  • To introduce and validate grating-coupled surface plasmon resonance (GCSPR) as a powerful tool for multiplexed, label-free analysis.
  • To demonstrate the capability of GCSPR for detecting various molecules of immunological interest and cellular components.
  • To highlight the potential of GCSPR in advancing the analysis of complex biological samples.

Main Methods:

  • Utilizing p-polarized light to excite surface plasmons on a gold sensor chip.

Related Experiment Videos

  • Immobilizing specific receptors (e.g., antibodies) on the sensor chip for analyte capture.
  • Measuring shifts in the grating-coupled surface plasmon resonance (GCSPR) angle, which correlate with refractive index changes upon analyte binding.

    • Main Results:

    • GCSPR enabled accurate, label-free assessment of analytes in a multiplexed format using small sample volumes.
    • The system demonstrated the capacity to analyze up to 400 analyte-receptor interactions on a single chip.
    • Simultaneous detection of cytokine arrays, proteins, and cellular analysis (antigen expression, apoptosis, T/B cell identification) was achieved.

    Conclusions:

    • Grating-coupled surface plasmon resonance (GCSPR) is a highly sensitive and multiplexed platform for label-free biomolecular and cellular analysis.
    • GCSPR technology offers a significant advancement for the comprehensive analysis of cells and molecular constituents in biological samples.
    • This method provides a powerful new approach for immunological studies and diagnostics.