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Updated: Aug 15, 2026

Volatile Sex Pheromone Extraction and Chemoattraction Assay in Caenorhabditis elegans
Published on: August 9, 2024
Sex attractant pheromone from the neotropical red-shouldered stink bug, Thyanta perditor (F.)
Maria C B Moraes1, Jocelyn G Millar, Raul A Laumann
1Embrapa Recursos Genéticos e Biotecnología, 02372, CEP 70849-970, Brasília, DF, Brazil.
Abstract:
Olfactometer bioassays showed that odors from mature Thyanta perditor males attracted females but not males. Furthermore, odors from females did not attract either sex, indicating that like other phytophagous pentatomid bugs, the males produce a sex pheromone. Attraction appeared to peak in late afternoon to evening. The headspace volatiles collected from male and female T. perditor were analyzed by GC-MS and HPLC. A male-specific compound, methyl (2E,4Z,6Z)-decatrienoate (2E,4Z,6Z-10:COOMe), was identified along with a number of other compounds found in extracts from both sexes. Bioassays carried out with 2E,4Z,6Z-10:COOMe showed it was as attractive to females as the crude extract of male volatiles, suggesting that the male-produced sex pheromone consists of 2E,4Z,6Z-10:COOMe as a single component. Consecutive volatiles collections from males showed that 2E,4Z,6Z-10:COOMe began appearing in extracts from males about 9 d after the final molt, as the males became sexually mature.
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