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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
A novel neutralizing monoclonal antibody against cell-binding polypeptide of ricin
Jian-Wei Guo1, Bei-Fen Shen, Jian-Nan Feng
1Department of Molecular Immunology, Institute of Basic Medical Sciences, Beijing, P.R. China., Clinical Laboratory, JinCheng Hospital, Lan Zhou, P.R. China.
Abstract:
One strain of neutralizing monoclonal antibody (MAb) against cell-binding polypeptide of ricin, named 3E1, was generated efficiently. The antibody recognized the linearity epitope of RTB located in a toxin structure domain characterized by Western blotting. The safe period of mice for intraperitoneal injection of 100 microg of antibody was 20 min after intraperitoneal injection of 2 microg of Ricin (10 times LD50). The neutralizing MAb we obtained could be developed into an immunotherapeutic agent to counteract the use of ricin as a terrorist or biological warfare weapon. It might be useful, as well, for antibody-based prophylaxis.
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