Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A case for chaperones in antigen processing.

D C DeNagel1, S K Pierce

  • 1Dept of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208-3500.

Immunology Today
|March 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Maladaptive health factors as potential mediators for the association between posttraumatic stress disorder and cardiovascular disease: A sex-stratified analysis in the U.S. adult population.

Journal of psychiatric research·2024
Same author

The Digestive Diverticula in the Carnivorous Nudibranch, <i>Melibe leonina</i>, Do Not Contain Photosynthetic Symbionts.

Integrative organismal biology (Oxford, England)·2021
Same author

On the Giant Octopus (Octopus giganteus) and the Bermuda Blob: Homage to A. E. Verrill.

The Biological bulletin·2017
Same author

Annual Viral Expression in a Sea Slug Population: Life Cycle Control and Symbiotic Chloroplast Maintenance.

The Biological bulletin·2017
Same author

The effects of the Epstein-Barr virus latent membrane protein 2A on B cell function.

International reviews of immunology·2002
Same author

The influence of CD40 on the association of the B cell antigen receptor with lipid rafts in mature and immature cells.

European journal of immunology·2001

Antigen-presenting cells assemble peptide-MHC-class-II complexes more efficiently than expected. Heat shock protein 70 family members may facilitate this crucial intracellular assembly process.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Peptide-MHC-class-II complex formation is essential for adaptive immunity.
  • In vitro studies show limited peptide binding to purified MHC class II molecules.
  • In vivo assembly within antigen-presenting cells appears significantly more efficient.

Purpose of the Study:

  • To investigate the discrepancy between in vitro and in vivo peptide-MHC-class-II assembly efficiency.
  • To explore the potential role of intracellular proteins in facilitating this process.
  • To present evidence for the involvement of heat shock protein 70 family members.

Main Methods:

  • The study focuses on presenting a case based on existing knowledge and experimental observations (specific methods not detailed in abstract).

Related Experiment Videos

  • Analysis of the intracellular environment of antigen-presenting cells.
  • Examination of the function of chaperone proteins, specifically heat shock protein 70.

    • Main Results:

    • The assembly of peptide-MHC-class-II complexes in antigen-presenting cells is significantly more efficient than predicted by in vitro binding assays.
    • Intracellular proteins within antigen-presenting cells are hypothesized to play a facilitative role.
    • Members of the chaperone/heat shock protein 70 family are implicated in promoting the intracellular assembly.

    Conclusions:

    • Heat shock protein 70 family members are likely involved in the intracellular assembly of processed-antigen-MHC-class-II complexes.
    • Chaperone proteins contribute to the enhanced efficiency of immune complex formation within antigen-presenting cells.
    • This finding offers a molecular explanation for the discrepancy observed in peptide-MHC-class-II assembly studies.