Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzymatic conversion-based method for screening cyclic dipeptide-producing microbes.

Panarat Arunrattiyakorn1, Teruhiko Nitoda, Hiroshi Kanzaki

  • 1Graduate School of Natural Science & Technology, Okayama University, Tsushima-naka, Okayama 700-8530, Japan.

Peptides
|October 18, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Solid-state fermentation of corn gluten meal with Aspergillus oryzae 6002 enhances amino acid and dipeptide accumulation while retaining high protein content.

Journal of bioscience and bioengineering·2026
Same author

Two new asteltoxins produced by solid-state fermentation of <i>Pochonia suchlasporia</i> TAMA 87 and their effects on cell division in sea urchin embryos.

Journal of pesticide science·2025
Same author

Solid-state cultivation of Aspergillus oryzae using insoluble plant cell wall polysaccharides and expression analyses of plant polysaccharide degradation-related enzymes.

Journal of bioscience and bioengineering·2025
Same author

Microbial biotransformation of proteins into amino acids in unpolished Thai and polished Japanese rice varieties cultivated with distinct industrial strains of koji mold.

Bioscience, biotechnology, and biochemistry·2025
Same author

Solid-state cultivation of multiple industrial strains of koji mold on different Thai unpolished rice cultivars: biotransformation of phenolic compounds and their effects on antioxidant activity.

Bioscience, biotechnology, and biochemistry·2024
Same author

Disease Burden and Progression in Patients with New-Onset Mild Cognitive Impairment and Alzheimer's Disease Identified from Japanese Claims Data: Evidence from the LIFE Study.

Journal of Alzheimer's disease : JAD·2023
Same journal

Potential role of intelectin-1 in the regulation of feeding of goldfish (Carassius auratus).

Peptides·2026
Same journal

Cocaine- and amphetamine-regulated transcript (CART) peptides: Neuroanatomical distribution and functional implications in the mammalian central nervous system.

Peptides·2026
Same journal

P1642-1, a novel pancreatic polypeptide analogue, ameliorates cognitive impairment in 5 ×FAD mice and is associated with enhanced PINK1/Parkin-related mitophagy.

Peptides·2026
Same journal

Structure-based rational design of high-affinity JAZF1 variants peptides to target the testicular orphan nuclear receptor 4 and pro-opiomelanocortin axis in Cushing's disease.

Peptides·2026
Same journal

[Gly<sup>2</sup>]-GLP-2(1-5): An ultra-short GLP-2 peptide for intestinal mucosal protection in inflammatory bowel disease.

Peptides·2026
Same journal

The role of Kiss1 neurons in regulating metabolism and energy balance.

Peptides·2026
See all related articles

We developed a novel enzymatic method to screen microbes producing cyclic dipeptides. This technique enabled the isolation of actinomycete strain A8, yielding cyclo(L-Phe-L-Pro) and cyclo(L-Pro-L-Tyr).

Area of Science:

  • Microbiology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Cyclic dipeptides (CDPs) are versatile compounds with diverse biological activities.
  • Efficient screening methods for CDP-producing microorganisms are crucial for discovering novel bioactive molecules.
  • Current methods often lack specificity or require extensive purification steps.

Purpose of the Study:

  • To develop a rapid and sensitive enzymatic method for screening cyclic dipeptide-producing microbes.
  • To isolate and identify novel CDP-producing microorganisms and their products.
  • To characterize the structures of purified cyclic dipeptides.

Main Methods:

  • Enzymatic conversion of CDPs to dehydro cyclic dipeptides using cyclo(Leu-Phe) oxidase.
  • Detection of dehydro derivatives via UV spectrophotometry using Thin Layer Chromatography (TLC) or High-Performance Liquid Chromatography (HPLC).

Related Experiment Videos

  • Enzymatic detection-guided fractionation for purification of CDPs from microbial extracts.

    • Main Results:

    • A novel screening method combining enzymatic conversion and spectrophotometric detection was established.
    • Actinomycete strain A8 was successfully isolated as a CDP-producing microorganism.
    • The purified CDPs from strain A8 were identified as cyclo(L-Phe-L-Pro) and cyclo(L-Pro-L-Tyr) using spectroscopic analysis.

    Conclusions:

    • The developed enzymatic screening method is effective for identifying CDP-producing microbes.
    • Actinomycete strain A8 represents a new source of valuable cyclic dipeptides.
    • This approach facilitates the discovery and production of novel cyclic dipeptides for potential applications.