Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Perfluorocarbon temperature measurements using 19F NMR.

B A Berkowitz1, J T Handa, C A Wilson

  • 1Department of Ophthalmology, UT South Western, Dallas, TX 75235-8592.

NMR in Biomedicine
|March 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stakeholder Support for Regulatory Harmonization and Expanded Nuclear Power: Outcomes of HPS/NCRP Open Forums.

Health physics·2026
Same author

Challenges and opportunities of the COVID-19 pandemic for perinatal mental health care: a mixed-methods study of mental health care staff.

Archives of women's mental health·2021
Same author

Increased risk of postpartum depression following severe obstetric lacerations in women with low resilience.

BJOG : an international journal of obstetrics and gynaecology·2020
Same author

Data for semi-permanent cationic coating for protein separations.

Data in brief·2020
Same author

Release of extracellular vesicle miR-494-3p by ARPE-19 cells with impaired mitochondria.

Biochimica et biophysica acta. General subjects·2020
Same author

Is there an increased risk of perinatal mental disorder in women with gestational diabetes? A systematic review and meta-analysis.

Diabetic medicine : a journal of the British Diabetic Association·2019

NMR chemical shift of perfluorotributylamine (FTBA) accurately measures temperature in vivo. This allows for precise oxygen tension (pO2) measurements using perfluorocarbons (PFCs) by correcting for temperature variations.

Area of Science:

  • Biomedical Engineering
  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Physiology

Background:

  • Perfluorocarbons (PFCs) enable in vivo oxygen tension (pO2) monitoring via fluorine NMR T1 measurements.
  • Temperature sensitivity of PFC T1 introduces errors in pO2 determination, especially in varying physiological conditions like ischemia.
  • Accurate tissue temperature measurement is crucial for reliable PFC-based pO2 assessment.

Purpose of the Study:

  • To evaluate the temperature dependence of the fluorine chemical shift of perfluorotributylamine (FTBA).
  • To establish FTBA's fluorine chemical shift as a reliable in vivo thermometer for correcting PFC pO2 measurements.
  • To report the first absolute in vivo temperature measurement using NMR.

Main Methods:

  • Investigated the in vitro linear relationship between FTBA fluorine chemical shift and temperature.

Related Experiment Videos

  • Assessed the independence of FTBA chemical shift from pO2.
  • Performed in vivo temperature measurements using an FTBA bubble in rabbit vitreous humor.
  • Compared NMR-derived temperatures with thermocouple measurements and core body temperature.

    • Main Results:

    • A linear correlation was observed between FTBA fluorine chemical shift and temperature in vitro.
    • FTBA chemical shift was found to be largely independent of pO2.
    • In vivo NMR temperature measurements in rabbit eyes agreed within +/- 0.5°C with thermocouple data.
    • NMR-determined temperatures closely matched core body temperatures.

    Conclusions:

    • FTBA fluorine chemical shift provides an accurate method for in vivo temperature determination.
    • This NMR-based thermometry method can correct for temperature-induced errors in PFC pO2 measurements.
    • The findings enable more accurate physiological monitoring using PFCs in diverse conditions.