Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Atopic ocular surface disease: implications on tear function and ocular surface mucins.

Murat Dogru1, Naoko Okada, Naoko Asano-Kato

  • 1Tokyo Dental College Ichikawa General Hospital, Department of Ophthalmology, Ichikawa, Japan. muratodooru@yahoo.com

Cornea
|October 18, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A whole-brain single-cell atlas of circadian neural activity in mice.

Science (New York, N.Y.)·2025
Same author

The Importance of High-Quality Cataract Surgery: A Case of Objective Improvement in Post-surgery Outcomes at a Rural Hospital in Vietnam.

Cureus·2025
Same author

Clinical course and efficacy of treatment with limbal-rigid contact lens wear for ocular sequelae in Stevens-Johnson syndrome/toxic epidermal necrolysis.

Japanese journal of ophthalmology·2025
Same author

Correlation Between Preoperative Aqueous Cytokine Levels and Mid-term Reduction of Corneal Endothelial Cells After Descemet Stripping Automated Endothelial Keratoplasty.

Cornea·2025
Same author

Impact of Intraoperative Descemet Membrane Perforation on Survival in Deep Anterior Lamellar Keratoplasty for Keratoconus.

Cornea·2025
Same author

Analysis of atmospheric particles washed away by eyewashes in Indonesia: The second report of the Jakarta study.

The journal of allergy and clinical immunology. Global·2025

Atopic eye disease causes ocular surface inflammation and tear film instability. Reduced MUC5AC mRNA expression is linked to noninfectious corneal shield ulcers in these patients.

Area of Science:

  • Ophthalmology
  • Immunology
  • Cell Biology

Background:

  • Atopic diseases can affect the ocular surface, leading to various disorders.
  • Understanding tear function and mucin alterations is crucial for managing atopic eye conditions.

Purpose of the Study:

  • To investigate tear function, mucin changes, and ocular surface abnormalities in patients with atopic diseases.
  • To correlate these findings with the presence of corneal ulceration.

Main Methods:

  • Corneal sensitivity, Schirmer test, and tear film break-up time (BUT) were assessed.
  • Ocular surface staining (fluorescein, rose Bengal) and cytology (impression, brush) were performed.
  • MUC5AC expression (protein and mRNA) was analyzed using immunohistochemistry and RT-PCR.

Related Experiment Videos

Main Results:

  • Atopic patients with corneal ulcers showed reduced corneal sensitivity and BUT compared to those without ulcers and controls.
  • Higher inflammatory cell expression was observed in corneal ulcers.
  • Eyes with ulcers exhibited squamous metaplasia, reduced goblet cells, and decreased MUC5AC mRNA expression.

Conclusions:

  • Ocular surface inflammation and tear film instability are key factors in atopic eye disease.
  • Diminished conjunctival MUC5AC mRNA expression plays a role in the development of noninfectious corneal shield ulcers.