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Published on: July 23, 2014
Characterization of lipoxygenase-1 null mutants in barley
1Bioresources Research and Development Laboratories, Sapporo Breweries Ltd., 37-1 Nitta-Kizaki, Ota, Gunma 370-0393, Japan. naohiko.hirota@sapporobeer.co.jp
Abstract:
This study describes the discovery and characterization of lipoxygenase-1 (LOX-1) null mutants in barley. Six lines did not exhibit any significant LOX activity in the silenced seed extract. Immunological analysis showed that these lines lacked the authentic LOX-1 protein. Genetic analysis of the F2 population revealed that this trait was governed by a single recessive gene located at the LoxA locus on chromosome 4H. The six LOX-1 null mutants shared similar features and the same unique polymorphism in a structural gene region, implying that these mutants might be derived from the same ancestral origin.
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