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TEM-induced gene mutations at enzyme loci in the mouse
Environmental Mutagenesis
|January 1, 1979
Summary
This study investigated triethylenemelamine (TEM) mutagenicity in mice, identifying four heritable mutations. Two mutations were spontaneous, while two others, Pgm-1 and Gpi-1, are presumed TEM-induced.
Area of Science:
- Genetics and Genomics
- Toxicology
- Mammalian Genetics
Background:
- Triethylenemelamine (TEM) is a chemical agent used to investigate mutagenic effects.
- Understanding induced mutations is crucial for assessing chemical safety and genetic risk.
- Mouse models are essential for studying heritable genetic alterations.
Purpose of the Study:
- To evaluate the mutagenic potential of triethylenemelamine (TEM) in mice.
- To identify and characterize induced mutations at specific enzyme loci in mouse offspring.
- To assess the viability and fertility of mice carrying identified mutations.
Main Methods:
- Male DBA/2J mice were treated with TEM and mated with C57BL/6J females.
- Starch gel electrophoresis was employed to screen F1 progeny for enzyme polymorphisms.
- Eleven specific enzyme loci were examined for the presence of induced mutations.
Main Results:
- Four heritable mutations were identified at the Es-1, Ldh-1, Pgm-1, and Gpi-1 loci.
- Mutations at Es-1 and Ldh-1 were found to be spontaneous, not TEM-induced.
- Presumed TEM-induced mutations at Pgm-1 and Gpi-1 were viable and fertile in heterozygotes, but homozygous offspring were not recovered.
Conclusions:
- TEM induced mutations at the Pgm-1 and Gpi-1 loci in mice.
- While heterozygous carriers of Pgm-1 and Gpi-1 mutations are viable, homozygosity appears to be lethal.
- Further research is needed to understand the genetic basis of lethality in homozygous mutants.