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Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
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Preparation and functional characterization of thylakoids from Arabidopsis thaliana

A P Casazza1, D Tarantino, C Soave

  • 1Dept of Biology, University of Milano, via Celoria 26, 20133, Milano, Italy, Carlo.Soave@unimi.it.

Photosynthesis Research
|October 18, 2005
PubMed

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Thylakoids are membrane-bound sac-like structures within the chloroplast that serve as sites for photosynthesis. Thylakoid lumen contains many electron transport proteins and is enclosed by a thylakoid membrane rich in the light-harvesting complex. Proteins targeted to the thylakoids are transported as precursors and are sorted by the general TOC/TIC import pathway. Once the precursor reaches the stroma, stromal processing peptidases remove their transit signal and expose thylakoid signal...