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[Expression specificity of hpt gene in sck transgenic rice]
Rui Wang1, Song-Biao Chen, Qin Zhuo
1National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Objective:
Evaluate the expression specificity of hpt gene in different developmental stages of sck transgenic rice.
Methods:
The different tissues, including leaves, culms, roots and seeds (ripening stage), in different developmental stages of sck transgenic rice were detected by PCR. The positive plants and the control plants were detected by Northern blot analysis and double sandwich ELISA test.
Results:
The hpt gene expression was found at transcription level and translation level in different tissues of sck transgenic rice. HPT protein in the seeds could not be detected.
Conclusion:
The hpt gene in different developmental stages of sck transgenic rice express stably at transcription level and translation level and HPT protein in the seeds could not be detected.
