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From assembly to virus particle budding: pertinence of the detergent resistant membranes

Anne-Sophie Gosselin-Grenet1, Geneviève Mottet-Osman, Laurent Roux

  • 1Department of Microbiology and Molecular Medicine, University of Geneva Medical School, CMU, 1 rue Michel-Servet, CH-1211 Geneva 4, Switzerland.

Virology
|October 19, 2005
PubMed

Insights

Sendai virus assembly in detergent-resistant membranes (DRMs) is not essential for virus production. Mutant proteins and M protein suppression experiments show DRMs do not directly precede virus budding, impacting infectivity but not particle numbers.

Area of Science:

  • Virology
  • Cell Biology
  • Membrane Biology

Background:

  • Detergent-resistant membranes (DRMs) are implicated as assembly sites for various viruses.
  • Understanding viral assembly mechanisms within cellular compartments is crucial for virology research.

Purpose of the Study:

  • To investigate the role of Sendai virus protein assembly in detergent-resistant membranes (DRMs) during virus particle production.
  • To determine if DRM-associated viral complexes are direct precursors for Sendai virus budding.

Main Methods:

  • Utilized Sendai virus mutant proteins that are not incorporated into virus particles.
  • Analyzed viral protein association with DRMs during infection.
  • Assessed the impact of M protein suppression on virus production and DRM association.
  • Investigated the effect of cellular cholesterol depletion on virus particle production and infectivity.

Main Results:

  • All Sendai virus proteins were found to associate with DRMs during infection.
  • Non-packaged mutant HN and M proteins also associated with DRMs.
  • Suppression of M protein significantly reduced virus production without altering other viral proteins' DRM association.
  • Cellular cholesterol depletion reduced virus infectivity but not virus particle production.

Conclusions:

  • The DRM-associated viral assembly complex is not a direct precursor for Sendai virus particle budding.
  • Virus particle production is independent of the specific DRM-based assembly complex investigated.
  • Cholesterol's role in Sendai virus may be more related to infectivity than particle formation.

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