Recombinant protein production in yeasts.

Area of Science:

• Biotechnology
• Molecular Biology
• Biochemistry

Background:

• Recombinant DNA (rDNA) technologies, encompassing genetic, protein, and metabolic engineering, are crucial for producing peptides, proteins, and biochemicals in non-native host cells.
• Established approximately 25 years ago, rDNA technologies represent a significant advancement in twentieth-century science and industry.
• Early successes of rDNA technology include the commercial production of pharmaceutical products and industrial enzymes.

Purpose of the Study:

• To review the advantages and limitations of various yeast hosts for heterologous protein production.
• To provide an overview of the most promising yeast systems currently available for biotechnological applications.

Main Methods:

• Literature review of established and emerging yeast expression systems.
• Comparative analysis of genetic, protein, and metabolic engineering strategies in yeast.
• Evaluation of yeast host performance for heterologous protein production.

Main Results:

• Yeast hosts offer significant advantages for producing heterologous proteins, despite advances in mammalian cell systems.
• Specific yeast strains and engineered systems demonstrate high potential for efficient protein and biochemical synthesis.
• Key limitations and challenges associated with yeast-based production systems are identified.

Conclusions:

• Yeast remains an attractive and versatile host for the industrial-scale production of a wide range of biomolecules using rDNA technology.
• Continued research and development in yeast engineering will further enhance its capabilities for biopharmaceutical and industrial enzyme manufacturing.
• Understanding the specific advantages and limitations of different yeast hosts is critical for optimizing recombinant protein production.