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Updated: May 25, 2026

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
Published on: September 2, 2014
[Relationship of microtubule and [Ca2+]cyt in wheat mesophyll protoplast]
1College of life science, Agricultural University of Hebei, Baoding 071001.
Abstract:
By using immunofluorescence labeling technique and incubating the Fluo-3AM of Ca2+ fluorescein, wheat (Triticum aestivum) cultivars Lovrin 10 was selected as mesophyll protoplast material in this paper for studying the relationship between the pattern of microtubule and [Ca2+]cyt. Laser scanning confocal microscopy(LSCM) was used and pharmacologic examination was set. The results indicated there was a connection between calcium signaling and microtubule organization in wheat mesophyll protoplast. When the cytosolic calcium concentration distinctly ascends, the pattern of microtubule showed depolymerization; Depolymerization of microtubule could enhance extracellar calcium afflux.
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