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Updated: Aug 15, 2026

RhoC GTPase Activation Assay
Published on: August 22, 2010
[Effect of RhoA and cAMP on morphological changes in human prostate cancer cell line PC-3]
Yong Chang Chen1, Ying Wang, Hao Yu
1School of Medicine, Jiangsu University, Zhenjiang 212001.
Abstract:
This paper was designed to investigate the effect of RhoA and cAMP/PKA mediating signal transduction on the morphological changes of human prostate cancer cell line PC-3. Different RhoA cDNA expressing constructs were used to transfect PC-3 cells. Transfected and untransfected cells were stimulated with lysophosphatidic acid(LPA) and/or cAMP. The morphological changes of the cells were recorded by inversive microscope. Results showed that the untransfected cells changed their conformation from polygonal to round with the stimulation of LPA,while cAMP prevented the change. LPA caused the same change in cells transfected with wild type, constitutively active type and 188 mutant of RhoA. cAMP prevented the change in cells transfected with wild type and constitutively active RhoA but not in cells transfected with 188 mutant of RhoA. The results suggested that cAMP/PKA could inhibit the morphological effect of RhoA through phosphorylating RhoA on 188 serine.
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