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[Construction of a full-length cDNA library of Aegilops speltoides Tausch with optimized cap-trapper method]

Xin-Guo Mao1, Xiu-Ying Kong, Guang-Yao Zhao

  • 1Key Laboratory of Crop Germplasm & Biotechnology/the Ministry of Agriculture, Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081, China. maoxg2001@sina.com

Yi Chuan Xue Bao = Acta Genetica Sinica
|October 20, 2005
PubMed

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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...