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Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
Published on: May 23, 2014
Nitric oxide induces endometrial secretion at implantation time
B Mörlin1, E Andersson, B Byström
1Department of Woman and Child Health, Division of Obstetrics and Gynecology, Karolinska University Hospital, Solna, Sweden. birgitta.morlin@kus.se
Acta Obstetricia Et Gynecologica Scandinavica
|October 20, 2005
Summary
Glandular nitric oxide (NO) production is essential for autonomic nervous control of endometrial secretion in guinea pigs. This finding suggests NO plays a crucial role during the implantation period.
Area of Science:
- Reproductive biology
- Endocrinology
- Neuroscience
Background:
- Sympathetic cholinergic innervation influences uterine cervical secretory cells.
- Nitric oxide (NO) is implicated in glandular secretion and early pregnancy.
- Implantation and early pregnancy share similarities with inflammatory responses.
Purpose of the Study:
- To investigate the role of nitric oxide (NO) in endometrial secretion.
- To determine if NO is a prerequisite for autonomic nervous modulation of endometrial secretion.
- To identify nitric oxide synthase (NOS) isoforms present during implantation.
Main Methods:
- Carbohydrate secretion from guinea pig uterine horns was measured in organ baths.
- Polymerase chain reaction identified NOS isoforms.
- Pharmacological agents modulated NO pathways and secretion.
Main Results:
- Carbamylcholine chloride (Carbachol) stimulated endometrial secretion; L-NNA and L-NAME inhibited this response.
- The NO donor GTN also induced secretion.
- Inhibiting soluble guanylyl cyclase (sGC) reduced responses to Carbachol and GTN.
Conclusions:
- Glandular NO production is necessary for autonomic nervous control of endometrial secretion in guinea pigs.
- NO likely plays a role in the process of implantation.
- Endothelial NOS (eNOS), inducible NOS (iNOS), and neuronal NOS (nNOS) are present at implantation time.
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