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Published on: October 10, 2017

Molecular cloning and characterization of two cDNAs encoding asparagine synthetase from Astragalus sinicus nodules

K Kasai1, M Fujie, Y Nakanishi

  • 1Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8527, Japan.

Journal of Bioscience and Bioengineering
|October 20, 2005
PubMed

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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...