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Published on: April 22, 2016
Mass production of D-psicose from d-fructose by a continuous bioreactor system using immobilized D-tagatose
K Takeshita1, A Suga, G Takada
1Department of Biochemistry and Food Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
Abstract:
An improved process for the mass production of D-psicose from D-fructose was developed. A D-fructose solution (60%, pH 7.0) was passed at 45 degrees C through a column filled with immobilized D-tagatose 3-epimerase (D-TE) which was produced using recombinant Escherichia coli, and 25% of the substrate was converted to D-psicose. After epimerization, the substrate, D-fructose, was removed by treatment with baker's yeast. The supernatant was concentrated to a syrup by evaporation under vacuum and D-psicose was crystallized with ethanol. Approximately 20 kg of pure crystal D-psicose was obtained in 60 d.
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