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Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
Optimization of xylanase production by Melanocarpus albomyces IIS68 in solid state fermentation using response
S Narang1, V Sahai, V S Bisaria
1Department of Biochemical Engineering and Biotechnology, Indian Institute of technology, Delhi, Hauz Khas, New Delhi-110016, India.
Journal of Bioscience and Bioengineering
|October 20, 2005
Summary
This study optimized conditions for xylanase production using Melanocarpus albomyces IIS68 fungus. Optimized solid-state fermentation yielded high xylanase activity, crucial for industrial applications.
Area of Science:
- Biotechnology
- Enzyme Technology
- Microbial Fermentation
Background:
- Xylanases are industrially important enzymes used in various sectors like food, feed, and biofuel production.
- Solid-state fermentation (SSF) offers an efficient method for enzyme production using fungal strains.
- Melanocarpus albomyces IIS68 is a thermophilic fungus with potential for high-value enzyme synthesis.
Purpose of the Study:
- To optimize solid-state fermentation conditions for enhanced xylanase production by Melanocarpus albomyces IIS68.
- To determine the optimal levels of key variables influencing enzyme yield.
- To validate the optimized conditions using statistical modeling.
Main Methods:
- Solid-state fermentation (SSF) of wheat straw using Melanocarpus albomyces IIS68.
- Response surface methodology (RSM) for optimizing fermentation variables.
- Multivariant statistical analysis to identify optimal parameter levels.
Main Results:
- Optimal conditions determined for particle size (600-850 µm), inoculum age (43 h), Tween 80 (1.37%), moisture content (86%), urea (5.1%), yeast extract (0.74%), and harvest time (96 h).
- Achieved a maximum xylanase activity of 7760 U/g initial dry substrate under optimized conditions.
- Experimental results closely matched the predicted value from the quadratic model (7890 U/g).
Conclusions:
- The study successfully optimized SSF parameters for high xylanase production by Melanocarpus albomyces IIS68.
- The optimized process demonstrates significant potential for cost-effective industrial-scale enzyme production.
- Further research could explore scale-up and application of the produced xylanase.
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