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Pancreastatin and bovine parathyroid cell secretion
1Calcium Endocrinology Laboratory, Department of Veterans Affairs Medical Center, Kansas City, MO 64128.
Bone and Mineral
|June 1, 1992
Summary
Bovine pancreastatin (bP32-47) inhibits parathyroid secretion of parathyroid hormone (PTH) and chromogranin A (CgA). However, this peptide is not detected in bovine parathyroid glands, questioning its physiological role.
Area of Science:
- Endocrinology
- Cell Biology
- Molecular Biology
Background:
- Chromogranin A (CgA) is a glycoprotein co-secreted with peptide hormones from secretory granules.
- Pancreastatin, a biologically active peptide, is derived from CgA.
Purpose of the Study:
- To investigate the effect of the C-terminal fragment of bovine pancreastatin (bP32-47) on bovine parathyroid cell secretion.
- To determine the presence and secretion of pancreastatin in bovine parathyroid glands.
Main Methods:
- Treatment of bovine parathyroid cells with bP32-47 amide.
- Measurement of parathyroid hormone (PTH) and CgA secretion.
- Analysis of CgA breakdown peptides and secretory granule extracts for pancreastatin.
Main Results:
- bP32-47 amide significantly inhibited low-calcium-stimulated PTH secretion by 44%.
- bP32-47 amide significantly inhibited CgA secretion by 33%.
- Pancreastatin was not detected in secretory granule extracts or incubation media, though a pancreastatin-like peptide was found as a minor CgA breakdown product.
Conclusions:
- Exogenous bovine pancreastatin inhibits parathyroid secretion of PTH and CgA.
- Detectable pancreastatin is not secreted by bovine parathyroid cells under normal conditions.
- The physiological importance of pancreastatin in bovine parathyroid glands is questionable based on current data.