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Gene amplification and its application in cell and tissue engineering.

Takeshi Omasa1

  • 1Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. omasa@bio.eng.osaka-u.ac.jp

Journal of Bioscience and Bioengineering
|October 20, 2005
PubMed
Summary

Gene amplification, the selective replication of specific genes, is crucial in eukaryotes for development and evolution. This review covers mammalian gene amplification, focusing on dihydrofolate reductase (dhfr) amplification in Chinese hamster ovary (CHO) cells for engineering applications.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Gene amplification is a widespread phenomenon in eukaryotes, involving selective replication of specific genes.
  • It plays a significant role in organismal development and evolution.
  • Mammalian gene amplification has notable applications in cell and tissue engineering.

Purpose of the Study:

  • To review mammalian gene amplification.
  • To discuss the dihydrofolate reductase (dhfr) gene amplification in Chinese hamster ovary (CHO) cells.
  • To explore the mechanisms, selection protocols, and applications of gene amplification.

Main Methods:

  • Literature review focusing on gene amplification in mammals.
  • Detailed examination of dhfr gene amplification in CHO cells.

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  • Analysis of gene amplification mechanisms and selection strategies.

    • Main Results:

    • Gene amplification is a key process in eukaryotes with implications for development and evolution.
    • The dhfr gene amplification in CHO cells serves as a model system.
    • Understanding gene amplification mechanisms and selection protocols is vital for its application.

    Conclusions:

    • Mammalian gene amplification is a powerful tool with significant applications in cell and tissue engineering.
    • Further research into gene amplification mechanisms can enhance its utility.
    • The dhfr system in CHO cells exemplifies the potential of gene amplification technologies.