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Related Experiment Videos

A modified ninhydrin reagent using ascorbic acid instead of potassium cyanide.

Sadaji Yokoyama1, Jun-Ichi Hiramatsu

  • 1Alcoholic Beverages and Food Research Laboratories, Takara Shuzo Co., Ltd., 3-4-1 Seta, Otsu-shi, Shiga 520-2193, Japan. yokoyamas@takara.co.jp

Journal of Bioscience and Bioengineering
|October 20, 2005
PubMed
Summary

A modified ninhydrin reagent using ascorbic acid offers a safer, economical method for amino acid photometric determination. This approach achieves a stoichiometric reaction and quantitative yield for glutamic acid analysis.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Ninhydrin reaction is a standard method for amino acid detection.
  • Potassium cyanide, a traditional reducing agent, poses toxicity concerns.

Purpose of the Study:

  • To investigate a modified ninhydrin reagent for amino acid photometric determination.
  • To evaluate ascorbic acid as a safer alternative to potassium cyanide.

Main Methods:

  • A modified ninhydrin reagent was prepared using ninhydrin, ascorbic acid, and methyl cellosolve.
  • The reagent was used for the photometric determination of amino acids, focusing on glutamic acid.

Main Results:

  • Ascorbic acid served as an effective, safe, and economical reducing agent.
  • An almost stoichiometric reaction with a quantitative yield of "Ruhemann's purple" was achieved for glutamic acid.

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Conclusions:

  • The modified ninhydrin reagent with ascorbic acid is suitable for amino acid photometric determination.
  • This method provides a safer and more cost-effective alternative to traditional reagents.