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Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna
Published on: February 1, 2022
Cloning, structural analysis and expression of the gene encoding aspartate aminotransferase from the thermophilic
Hyeung Kim1, Masaki Nakaoka, Mayumi Yagi
1Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 69028504, Japan.
Journal of Bioscience and Bioengineering
|October 20, 2005
Abstract:
The aspartate aminotransferase gene from the thermophilic cyanobacterium Phormidium lapideum was cloned and expressed in Escherichia coli. The ORF of 1167 nucleotides encodes a protein of 388 amino acids having a molecular weight of 42,099. A molecular model of PIAspAT shows structural features similar to those of the Thermus thermophilus AspAT.
