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Updated: Aug 15, 2026
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
Published on: November 12, 2012
Selection of a suitable strain from recombinant Escherichia coli strains with the same genetic structure expressing
Seyed Safa-Ali Fatemi1, Bagher Yakhchali, Jafar Towfighi Darian
1Industrial Fermentation Group, National Research Center for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, Iran.
Abstract:
The selection of a suitable strain among five recombinant Escherichia coli strains with the same genetic structure that expresses the human granulocyte macrophage colony stimulating factor (hGM-CSF) was carried out based on four criteria:growth rate, expression level, plasmid stability and feasibility for protein extraction. There was no significant difference in growth between the five strains, while a suitable expression level, a high plasmid stability and a good feasibility for protein extraction from periplasmic space were observed for one of the recombinant strains. This strain expressed 27% hGM-CSF relative to total proteins and had 96% plasmid stability after 7-d subcultures on an antibiotic-free LB medium.
