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Updated: Aug 15, 2026

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
Electrochemical detection of nonlabeled oligonucleotide DNA using biotin-modified DNA(ss) on a streptavidin-modified
Jong Wan Park1, Hea-Yeon Lee, Jong Min Kim
1The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
Abstract:
In the electrochemical detection of nonlabeled DNA, it is important to control the bonding at the interface between the DNA and the electrode. Atomic force microscope (AFM) was taken for the commonly used thiol-modified DNA on a gold surface. It was found that the coverage of the DNA was very low. On the other hand, a streptavidin-modified gold electrode provided a much better alternative where DNA hybridization resulted in large changes in the electrochemical reaction responses. This work demonstrates that streptavidin-modified gold electrodes could be used in the development of a new electrochemical protocol for the detection of nonlabeled DNA.
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