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Updated: Aug 15, 2026

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
Published on: January 15, 2014
Effect of freeze-thaw repetitions upon the supercooling release ability of ice-nucleating bacteria
Yooko Tsuchiya1, Kazuhiro Sasaki, Hiromi Hasegawa
1Energy & Mechanical Engineering Department, Central Research Institute of Electric Power Industry, 2-6-1 Nagasaka, Yokosuka, Kanagawa 240-0196, Japan. yooko@criepi.denken.or.jp
Abstract:
We have studied the durability of ice-nucleating bacteria with a potent supercooling release capacity through repeated freeze-thaw cycles. Through experiment, we confirmed that UV sterilized Erwinia ananas maintains a superior supercooling release capacity at around -1 degrees C through 2000 freeze-thaw cycles. We also found that gamma-ray sterilization, which is more suitable than UV for large-scale sterilization treatment, has a similar effect at appropriately selected doses.
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