Related Experiment Video
Updated: Aug 15, 2026

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
Published on: January 15, 2014
Production of two types of ice crystal-controlling proteins in Antarctic bacterium
Hidehisa Kawahara1, Yoko Nakano, Kazuhiro Omiya
1Department of Biotechnology, Faculty of Engineering, Kansai University, 3-3-35 Yamate-cho, Suita-shi, Osaka 564-8680, Japan. kawahara@ipcku.kansai-u.ac.jp
Abstract:
Pseudomonas fluorescens KUAF-68, which was isolated from Antarctica, had both ice-nucleating protein and antifreeze protein activities in the culture broth. We found that both proteins were separately produced based on the results of column chromatography, SDS-PAGE analysis and Southern hybridization. The activity of the ice-nucleating protein was stimulated by the addition of glycine (0.020 N%), whereas the activity of the antifreeze protein was stimulated by the addition of L-asparagine (0.025 N%). This is the first report on the production of two types of ice crystal-controlling proteins in one bacterial strain.
More Related Videos
Related Concept Videos
Cytoskeletal Proteins in Bacteria
Surface Appendages of Archaea
Diversity of Archaea III
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Antimicrobial Proteins
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Bacterial Protein Maturation

