Constructing an amylolytic brewing yeast Saccharomyces pastorianus suitable for accelerated brewing
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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Zengran Liu1, Guangyi Zhang, Shigui Liu
1Bioscience and Bioengineering College, Hebei Economics and Business University, 47 Xuefu Road, Shijiazhuang 050061, PR China.
Abstract:
An amylolytic brewing yeast Saccharomyces pastorianus, free of vector sequences and drug-resistance genes, was constructed by disrupting the alpha-acetolactate synthase gene and introducing the alpha-amylase gene as a selective marker. The resulting recombinant strain was able to utilize starch as the sole carbon source and its alpha-acetolactate synthase activity was lowered by 30%. Fermentation tests confirmed that the diacetyl concentration and the residual oligosaccharide were reduced by 70% and 25%, respectively, in fermented wort by the recombinant strain, while the brewing performance of the recombinant strain was retained.
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