Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydrostatic pressure/perfusion culture system designed and validated for engineering tissue.

Setsuo Watanabe1, Shuji Inagaki, Ibuki Kinouchi

  • 1Medical Group, Takagi Industrial, 104 Nishi-kashiwabara-shinden, Fuji, Shizuoka 417-8505, Japan. wata0472@purpose.co.jp

Journal of Bioscience and Bioengineering
|October 20, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cannonballs in <i>Trichomonas vaginalis</i> Infection: Morphologic Evidence of Parasite-Associated Neutrophilic Aggregates.

Pathogens (Basel, Switzerland)·2026
Same author

Koilocytosis in LSIL Cytology Has Limited Predictive Value for CIN2+ in HPV-Positive Women: Implications for Risk-Based Cytology Triage.

Pathogens (Basel, Switzerland)·2026
Same author

Vacuolated Parabasal Cells in Papanicolaou Smears Are Cellular Changes Caused by Human Papillomavirus 16 Infection.

Journal of medical virology·2025
Same author

Effects of hydrostatic pressure, osmotic pressure, and confinement on extracellular matrix associated responses in the nucleus pulposus cells ex vivo.

Matrix biology : journal of the International Society for Matrix Biology·2024
Same author

Bidirectionally validated <i>in silico</i> and <i>in vitro</i> formation of specific depth zone-derived chondrocyte spheroids and clusters.

Frontiers in bioengineering and biotechnology·2024
Same author

Cellular behavior and extracellular matrix turnover in bovine annulus fibrosus cells under hydrostatic pressure and deviatoric strain.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2023

A new hydrostatic pressure (HP)/perfusion system supports three-dimensional cell construct viability for tissue engineering. This validated system enables controlled mechanical stimuli, crucial for regenerating damaged tissues.

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Tissue engineering aims to regenerate damaged tissues using three-dimensional cell constructs.
  • Maintaining cell viability and phenotype in vitro is critical for successful tissue regeneration.
  • Existing methods for cell construct production require improved control over the in vitro environment.

Purpose of the Study:

  • To develop and validate a novel hydrostatic pressure (HP)/perfusion culture system for three-dimensional cell constructs.
  • To assess the system's ability to apply mechanical stimuli through controlled HP and medium perfusion.
  • To evaluate the impact of HP on air bubble dynamics within the culture system.

Main Methods:

  • Development of a hydrostatic pressure (HP)/perfusion culture system for 3D cell constructs.

Related Experiment Videos

  • Testing system stability under constant HP (up to 5 MPa) and cyclic HP (0-5 MPa at 0.5-0.03 Hz).
  • Analysis of medium perfusion flow patterns and investigation of air bubble behavior under HP using a 3D agarose gel model.

    • Main Results:

    • The HP/perfusion system demonstrated stable performance at constant and cyclic HP within tested ranges.
    • Medium perfusion exhibited laminar flow, ensuring consistent nutrient and waste exchange.
    • Hydrostatic pressure effectively eliminated air bubbles in the 3D agarose gel model, replacing them with culture medium.

    Conclusions:

    • The validated HP/perfusion system provides well-regulated hydrostatic pressure application for 3D cell cultures.
    • This system is suitable for applying mechanical stimuli to enhance cell viability and phenotype in tissue engineering.
    • The technology holds potential for broader applications in 3D tissue culture and regenerative medicine.