Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydrodynamic filtration for on-chip particle concentration and classification utilizing microfluidics.

Masumi Yamada1, Minoru Seki

  • 1Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.

Lab on a Chip
|October 20, 2005
PubMed
Summary

We introduce hydrodynamic filtration, a new microfluidic technique for continuous particle concentration and classification. This method efficiently separates particles by size without clogging, enabling applications in cell sorting and analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Does Aquablation Therapy Improve Lower Urinary Tract Symptoms in Patients Aged ≥ 75 Years With Benign Prostatic Obstruction?

Lower urinary tract symptoms·2026
Same author

Vertical-flow porous microchamber arrays for cell capture, intracellular molecule staining, and population analysis.

RSC advances·2026
Same author

Comparison of surgical outcomes of robot-assisted radical prostatectomy between residents under our training curriculum and experienced surgeons: a propensity score-matched analysis.

International journal of clinical oncology·2026
Same author

Optimizing Patient Selection for Aquablation During the Initial Adoption Phase: Prostates Smaller Than 100 mL.

International journal of urology : official journal of the Japanese Urological Association·2026
Same author

Surgical anatomy of accessory pudendal arteries in Japanese men: preservation techniques for robot-assisted radical prostatectomy.

Asian journal of urology·2025
Same author

Constriction-induced capture of rare cells using interdigitated microchannels with bidirectionally perforated thin honeycomb films.

Talanta·2025

Area of Science:

  • Biotechnology
  • Microfluidics
  • Particle Science

Background:

  • Microfluidic devices offer precise control over small fluid volumes.
  • Continuous particle concentration and classification are crucial for various applications, including diagnostics and cell sorting.
  • Existing methods often face challenges like channel clogging and limited throughput.

Purpose of the Study:

  • To develop a novel method for simultaneous continuous particle concentration and classification in microfluidic devices.
  • To demonstrate the efficacy of hydrodynamic filtration for particle separation based on size.
  • To validate the method's applicability to biological samples like blood.

Main Methods:

  • Hydrodynamic filtration utilizes microchannels with side branch channels to concentrate particles along sidewalls.

Related Experiment Videos

  • Particle concentration is achieved by withdrawing liquid through side channels, aligning particles based on their inability to approach sidewalls.
  • Downstream selection channels enable size-based collection of concentrated particles.

    • Main Results:

    • Achieved 20-50-fold concentration of polymer microspheres (1-3 micrometers).
    • Demonstrated independent collection of particles based on their size.
    • Successfully performed selective enrichment of leukocytes from blood samples.

    Conclusions:

    • Hydrodynamic filtration is an effective technique for continuous particle concentration and size-based classification in microfluidics.
    • The method avoids channel clogging, even with large channel widths relative to particle size.
    • This technique shows promise for applications in biological sample processing and analysis.