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Wen-Tso Liu1, Liang Zhu, Qi-Wei Qin

  • 1Division of Environmental Science and Engineering, National University of Singapore, Blk E1A, #07-03, Engineering Drive 2, Singapore 117576. cveliuwt@nus.edu.sg

Lab on a Chip
|October 20, 2005
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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...